Pack Size : 96 Well

Package Kit Components :

• Microwells  coated
• Wash Solution
• Anti-HBs HRP Conjugate
• Anti HBs HRP
• TMB Substrate
• TMB Diluent
• Stop Solution
• Positive Control
• Negative Control
• Technical Insert

Usage : New Life  HBsAg ELISA Test is an in vitro enzyme immunoassay for the detection of hepatitis B surface antigen in  human serum or plasma. The HBsAg ELISA is primarily used for testing Blood and organ donors, patients with active viral hepatitis and people at risk for hepatitis B infection.

Description: Hepatitis B virus is responsible for the most serious form of the hepatitis related diseases. HBV is transmitted through Sexual contact blood and other body fluids transmitted from an infected mother to her child during the perinatal period. Hepatitis B Surface Antigen (HBsAg) is usually detectable around two to six weeks in advance of clinical and biochemical evidence of hepatitis and persits throughout the clinical course of the disease. Level of HBsAg is of maximum titer at the height of liver damage. HBsAg is a complex lipid and glycoprotein particle containing group specific determinants‘d/y’ and ‘w/r’. thus HBsAg can be subdivided into four major antigenic subtypes : 'adw', 'adr', 'ayw', and 'ayr'. The subtype 'ayw' is predominant in India. Hepatitis B Virion (HBV) or Dane particle is a double-stranded virus which is 42nm in diameter with a lipoprotein coat and a nucleocapsid core. The lipoprotein coat is composed of Hepatitis B surface antigen (HBsAg). The nucleocapsid is 27nm in diameter and possesses a distinct antigenic specificity (HBsAg) along with a partially double stranded DNA molecule and an endogenous polymerase enzyme. Another antigen called “Hepatitis B” e antigen (HBeAg) is a split protein of HBsAg. All the above antigen and viral DNA polymerase form useful diagnostic markers for HBV. However, HBsAg has been accepted as a universal and the most reliable seromarker in case of acute HBV infection due to its utmost appearance almost 2-4 weeks before the ALT level becomes abnormal and 3-5 weeks before the onset of symptoms or jaundice as an early detection system for hepatitis B virus infection. Of symptoms or jaundice as an early detection system for hepatitis B virus infection.