Pack Size : 96 Well

Package Kit Components :

• Microwells  coated
• Dilution buffer
• Wash Solution
• HRP Conjugate
• HRP Conjugate diluent
• TMB Substrate
• TMB Diluent
• Stop Solution
• Positive Control
• Negative Control
• Technical Insert

Usage : The  HCV  ELISA  Test  is for the in vitro detection of antibodies to Hepatitis C Virus (HCV) in human serum or Plasma. It is intended as a screening test for donated blood to prevent transmission of HCV to recipients and as an aid in clinical diagnosis of HCV related infections.

Description : Hepatitis C Virus (HCV) is a small, enveloped, positive-sense single stranded RNA Virus. HCV is known to be the major cause of parenterally transmitted non-A, non-B, (NANB) hepatitis. Like the hepatitis B virus, HCV is typically transmitted parenterally. It is associated especially with the transfusion of contaminated blood and blood products. Other common routes of transmission include intravenous drug abuse and needle stick accidents. Present evidence indicates that sexual route is not important in the transmission of HCV. However, HCV transmission occurs more readily and with greater frequency if the sexual partner is co-infected with HIV. Prenatal transmission of HCV from mother to infant is uncommon. The risk of mother to infant transmission may be much greater if the mother is co-infected with HIV. The risk of post transfusion hepatitis was estimated to be 7 to 18%, with approximately 90% of post-transfusion hepatitis being caused by the NANB hepatitis agent. Conventional methods failed to isolate the virus in cell culture or visualize it by electron microscope. Cloning the viral genome made it possible to develop serological assays that use recombinant antigens. Compared to the first generation HCV Elisa using recombinant antigen, multiple antigen using recombinant protein and synthetic peptides have been added in new serological tests (Third generation tests ) to avoid non specific cross reactivity and to  Increase the sensitivity of the HCV antibody tests. The test for antibodies to HCV was proved to be highly valuable in the diagnosis and study of the Infection, especially in the early diagnosis of HCV after transfusion. The Diagnosis of hepatitis C can be easily made by finding elevated serum ALT Levels and presence of anti-HCV in serum or plasma. The HCV GENOME ENCODES FOR THREE STRUCTURAL PROTEINS (Capsid protein an Seeral non- structural proteins (NS2,NS3,NS4 & NS5).