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Pack Size : 96 Well
Package Kit Components :
Usage : Vitamin Folic Acid Test is a microtiter plate test kit based on a microbiological assay which measures the total folic acid content in serum. .
Description Serum samples are diluted with a buffer solution. The diluted samples are added into the microtiter plate wells [coated with Lactobacillus rhamnosus which metabolizes folic acid. The addition of folic acid in either standards or samples gives a folic acid-dependent growth response until it is consumed. After incubation at 37°C for 48 h, the growth of Lactobacillus rhamnosus is measured turbidimetrically at 610 - 630 nm (alternative at 540 - 550 nm) in an ELISA-reader and a standard curve is generated from the dilution series. The amount of folic acid is directly proportional to the turbidity.
