Pack Size : 96 Well

Package Kit Components :

• Microwells  coated
• Sperm Antibody ELISA standard set
• Dilution buffer
• Control
• Enzyme Conjugate
• Substrate Solution
• Stop Solution
• Wash Solution
• Technical Insert

Usage : The Sperm Antibody ELISA is a reliable and quantitative test for the determination of antibodies directed against human spermatozoa. This test is intended for the use with serum.

Description The Sperm Antibody ELISA (Enzyme Linked Immunosorbent Assay) is a solid-phase sandwich enzyme-immunoassay for the quantitative determination of anti-spermatozoa antibodies in human serum. The ELISA-plate is coated with a mix of spermatozoa proteins which are recognized by anti-spermatozoa antibodies. The samples and standards are pipetted into the wells and then incubated. During this incubation anti-spermatozoa antibodies bind to the spermatozoa proteins and are thus immobilised on the plate. After washing the enzyme conjugate, consisting of anti-human globulin antibodies covalently coupled to horseradish peroxidase, is added. After removal of the unbound conjugate by washing the horseradish peroxidase oxidizes the then added substrate TMB (3,3’,5,5’-tetramethylbenzidine) yielding a colour reaction which is stopped with 0.25 M sulphuric acid (H2SO4). The extinction is measured at a wavelength of 450 nm with a microplate reader. The use of a reference measurement with a wavelength ≥550 nm is recommended.