Pack Size : 96 Well

Package Kit Components :

• Microwells coated
• PTH Standards
• PTH Controls
• Anti-PTH Biotin Regent
• Anti-PTH-HRP-Enzyme Conjugate
• TMB Substrate
• Stop Solution
• Wash concentrate
• Technical Insert

Usage : The New Life Intact –PTH Elisa kit is intended for thee quantitative determination of Intact –PTH Elisa  in human serum

Description : PTH (Parathyroid hormone) is biosynthesized in the parathyroid  gland as a pre-proparathyroid hormone, a large molecular precursor consisting of 115 amino acids. After two intra-cellular proteolytic cleavage steps, the parathyroid gland secretes the final active form consisting of an 84 amino acid peptide. In healthy individuals, regulation of parathyroid hormone secretion normally occurs via a negative feedback action of serum calcium on the parathyroid glands. intact PTH is biologically active and clears very rapidly from the circulation with a half-life of less than four minutes. Intact PTH assays are important for the differentiation of primary hyperparathyroidism from other (non-parathyroid-mediated) forms of hypercalcemia, such as malignancy, sarcodosis and thyrotoxicosis. The measurement of parathyroid hormone is the most specific way of making the Diagnosis of primay hyperparathyroidism. ln the presence of hypercalcemia, an elevated level of parathyroid hormone virtually establishes the diagnosis. in over 90% of patents with primary hyperparathyroidism, the parathyroid hormone will be elevated. The most common other cause of hypercalcemia, namely hypercalcemia of malignancy, is associated with suppressed levels parathyroid hormone or PTH levels within the normal range. PTH values are typically undetectable in hypocalcemia due to total hypoparathyroidism, but are found within the normal range in hypocalcmia due to partial loss or inhibition of parathyroid function.